2024 Gapdh antibody dilution

Gapdh antibody dilution

Author: geww

August undefined, 2024

WebIn Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. ... Our GAPDH antibody NB300-221 has been validated in simple western and detects around 35 kDa so I think it would be a good choice for a simple western loading control antibody based on your size requirements. WebThe GAPDH (glyceraldehyde-3-phosphate dehydrogenase) gene is mapped to human chromosome 12p13.3 and encodes a tetramer containing identical chains. 1] The enzyme is commonly known as glycolytic enzyme and contains a binding site for NAD + (nicotinamide adenine diphosphate) and glyceraldehyde-3-phosphate. GAPDH contributes to 10-20% …

Anti-beta Actin antibody (ab8227) Abcam

WebMonoclonal Antibody for studying GAPDH. Cited in 5519 publications. Validated for WB, WB, IHC, IF. Available in 2 sizes. Highly specific and rigorously validated in-house, GAPDH (D16H11) XP® Rabbit … WebGAPDH was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181602 at 1/60 dilution. Western blot was … office 365 terminal server lizenz

Western blotting results: No GAPDH? ResearchGate

Web11 rows · Anti-GAPDH, Cat. No. AB2302, is a chicken polyclonal antibody that detects Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and is tested for use in Western … WebWestern blot analysis of GAPDH in A) PC-12 whole cell lysate, B) rat2 whole cell lysate using GAPDH polyclonal antibody (Product # PA5-85074) using 30 µg of sample at a dilution of 1:10000. Sample was then incubated with HRP-conjugated anti-rabbit IgG secondary antibody. ... Proteins were transferred to a membrane and probed with a … WebThere are 12 GAPDH antibodies which are validated in multiple tissues with various applications, including WB, IHC-P, ELISA, IP, ICC/IF. There are 9 GAPDH antibody for WB, 4 GAPDH antibody for IHC-P, 3 GAPDH antibody for ELISA, 4 GAPDH antibody for IP, 2 GAPDH antibody for ICC/IF. Among all these GAPDH antibodies, there are 3 anti … office 365 tender

GAPDH (D4C6R) Mouse mAb Cell Signaling Technology

GAPDH (14C10) Rabbit mAb Cell Signaling Technology

WebThe section was then incubated with ab8227, 1/1000 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody (ab6720, 1/1000 dilution) was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. WebApr 1, 2016 · Brian Gregory George Oliver. Western blot analysis of A: PDE3B (n = 4 non-asthmatic and n = 4 asthmatic) and B: PDE5A (n = 4 non-asthmatic and n = 4 asthmatic) expression in non-asthmatic and ... mychart login prime healthWebMonoclonal Anti-GAPDH antibody produced in mouse is suitable for western blotting using: protein extracted from heart tissue of mice at a working dilution of 1:25,000 3 myelin … office 365 terminated employee mailbox

"WebGAPDH (Glyceraldehyde-3-phosphate dehydrogenase) is a catalytic enzyme commonly known to be involved in glycolysis. GAPDH exists as a tetramer of identical 37-kDa subunits and catalyzes the reversible reduction of 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis ... " - Gapdh antibody dilution

Gapdh antibody dilution

GAPDH antibody (HRP-60004) Proteintech - ptglab

WebRabbit anti Human GAPDH (AHP1628) was used as the primary antibody at a dilution of 1/1000. Mouse anti Rabbit Light Chain:HRP MCA6003P was used as the secondary … WebThe cells were labeled with GAPDH Mouse Monoclonal Antibody (Product # AM4300) at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with …

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WebRecommended dilution: WB 1:10000 - 1:760000; IHC 1:100 - 1:500; IF/ICC 1:50 - 1:200; Antibody Dilute Procedure: Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted … WebThe membrane was probed with a GAPDH polyclonal antibody (Product # PA1-987) at a dilution of 1:3000 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room …

WebMy GAPDH is a rabbit polyclonal antibody, using dilution of 1:20000. But for secondary antibody, I used 1:5000. Sometimes I wonder would it be the cross-activity across the species. WebAnti-GAPDH Antibody. Anti-Glyceraldehyde-3-Phosphate Dehydrogenase Antibody is popular affinity purified polyclonal antibody. This antibody is supported by peer reviewed publications and reliably detects Glyceraldehyde-3. Anti-GAPDH Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available …

WebGet better batch-to-batch reproducibility with a recombinant antibody Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) Research with confidence – … WebGAPDH antibody used for WB at 1:1000 dilution on lysates of mouse embryonal carcinoma P19 cell line which was induced to differentiate for 5 days while being …

WebAll lanes : Anti-GAPDH antibody [EPR6256] - Loading Control (ab128915) at 1/10000 dilution ... (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor ® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary ...

WebImmunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10494-1-AP (GAPDH antibody) at dilution of 1:400 (under 10x lens. Heat mediated … mychart login portland orWebGAPDH作为管家基因在同种细胞或者组织中的蛋白质表达量一般是恒定的，且几乎在所有组织中都高水平表达，因此被广泛用作Western blot蛋白质标准化的内参。. 产品编号. 产品名称. 包装. AG0122. GAPDH Rabbit Monoclonal Antibody. 50µl. AZ050. Western一抗稀释液. mychart login ppgWebA specific band was detected for GAPDH at approximately 43 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat GAPDH Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5718) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing … mychart login poplar bluff moWeb(sc-32233) GAPDH Antibody (6C5) Supplier Santa Cruz Biotechnology. Host Mouse Type Primary Clonality Monoclonal (6C5) Conjugate Unconjugated Target Glyceraldehyde-3-phosphate dehydrogenase (Oryctolagus cuniculus) ... Dilutions for 3,560 citations have been found for this ... office 365 terminal server lizenzierungWebImmunocytochemistry analysis of GAPDH in HeLa cells. Samples were incubated in GAPDH monoclonal antibody ( Product # MA1-16757) using a dilution of 1:100. … mychart login prohealth care nyWebWestern Blot: GAPDH Antibody [NB300-327] - Analysis of different cell cytosolic or nuclear enriched fractions, GAPDH antibody, dilution 1:20,000 (Red): [1] protein standard, [2] … mychart login prisma greenvilleWebThe cells were labeled with GAPDH Rabbit Polyclonal Antibody (Product # PA1-16777) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature ... office 365 terms of service