WebFor subcellular fractionation, about 1 x 10 5 cells were seeded in 60 mm Petri dishes, and then the cytosolic proteins were extracted adding buffer 1 (10 mM HEPES, pH 7.9, 50 mM NaCl, 0.5 M sucrose, 0.1 mM EDTA, 0.5% Triton X-100) and supplemented with 0.1 mM DTT, 10 mM NaF, 10 mM NA 3 VO 4, 1 mM PMSF and protease inhibitor (SigmaFast, … WebSIGMAFAST ™ Protease Inhibitor Tablets are a water-soluble tablet formulation designed to inhibit serine, cysteine, aspartic, and metalloproteases. The tablets contain a broad …

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