2024 Strain bj3505

Strain bj3505

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Web1 Oct 2010 · BJ3505 CBP-Vam3p nyv1Δ was used for trans-SNARE complex isolation (Collins and Wickner, 2007). NHX1 was deleted from BJ3505, DKY6281 and BJ3505 CBP … WebDownload scientific diagram Extra- and intracellular β -galactosidase production by S.cerevisiae strain BJ3505 transformed with plasmid YEpFLAG1-LAC4 ( A) or YEpFLAG1-BC ( B) growing in YPHSM ...

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Web21 Aug 2007 · Standard fusion and lysis reactions (×16 scale) bore vacuoles from BJ3505 GFP and DKY6281 or 4SNARE-overproducing strains BJ3505 4SNARE ++ GFP, DKY6281 … WebThe protein Ixr1p fused to the FLAG ™ peptide at the carboxyl-terminal end was expressed in the yeast strain BJ3505 . Cells were grown at 30 °C beyond 48 h in YPHSM medium (8% Bacto peptone, 1% yeast extract, 3% glycerol and 1% dextrose) until OD 600 nm 6.0, coinciding with the maximum expression. Cells were lysed in TBS buffer (50 mM Tris ... second infantry division birthday

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Web1 Mar 2000 · A BJ3505 wild-type culture was processed for vacuole purification . An aliquot of spheroplasted yeast cells was lysed by boiling in SDS-PAGE loading buffer, and the … Web1 Dec 2012 · BJ3505 CBP (calmodulin-binding peptide)–Vam3 nyv1Δ was used for trans-SNARE complex isolation (Table 1). YBT1 was deleted from BJ3505, DKY6281 and … Web13 Oct 2024 · Trans-SNARE pairing was analyzed with vacuoles prepared from DKY6281 vam3Δ, BJ3505 nyv1Δ, and BJ3505 nyv1Δ VAM3ΔN. Vacuoles (120 μg each) were mixed … second industry

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WebThe strains BJ3505ΔSuc2 and BJ3505 were transformed with plasmids YEpFLAG-1, YEpMEL1, and YEpMEL1His, by a modification of the lithium acetate method (Chen et al., … Web27 Jul 2015 · In brief, strain BJ3505 was grown to mid-log phase in 4 liters of YPD medium. Cells were harvested, washed once with PS buffer containing 150 mM KOAc and 5 mM MgOAc, resuspended in the same buffer containing 1 mM PMSF and 10 µM leupeptin, and quick-frozen by drop-wise addition to liquid nitrogen. The frozen cells were mixed with … punk with a cameraWeb1 May 2001 · The Saccharomyces cerevisiae BJ3505 strain was transformed with these four plasmids (YEpFLAG1-LAC4, YEpFLAG1-BC, YEpFLAG1-BD, YEpFLAG1-EF). β … second industrial revolution in germany

"Web1 Dec 2001 · Vacuolar morphology is unaltered in the Vam3N mutant. Wild-type or mutant BJ3505 strains were incubated with 10 M of the dye FM4-64 (Molecular Probes, Eugene, OR) for 20 min at 30°C in YPD. " - Strain bj3505

Strain bj3505

VacuolarSNAREProteinTransmembraneDomainsServeas ...

Web15 Aug 2000 · Frozen BJ3505 vacuoles (300 μg) were diluted to 1.5 ml with PS buffer (see Materials and Methods) and sedimented (13,000 × g, 10 min, 4°C). Vacuole pellets were … WebUsing different Rhizopus sp. strains as fermentation agents, this paper describes a global strategy to identify interactions between cultivation parameters (pH 4.75–7.75, 7.5–82.5 g/l glucose ...

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WebThe strain BJ3505 carries the wild-type PHO8 gene but lacks genes encoding the major vacuolar proteases that activate the Pho8p phosphatase. Thus, vacuoles isolated from … Web1 Nov 2001 · The strains BJ3505 VPS33::ProtA and BJ3505 VAM3ΔN VPS33::ProtA were created by transformation of a PCR fragment containing the sequence encoding Protein A and a kanMX6 selection marker with flanking regions of the VPS33 3′-region and the downstream sequence of the terminator ( Knop et al., 1999 ).

Web1 Aug 2000 · Vacuole Isolation. Vacuoles were isolated from yeast strains BJ3505 and DKY6281 as described ().Large-scale frozen vacuole preparations of BJ3505 vacuoles were used for studies of glutathione S-transferase (GST)-Ypt7p association and for immunoprecipitation experiments. Spheroplast pellets were resuspended in an equal … WebDownload scientific diagram Monitoring of variants MEL1 expressed in strain BJ3505 using YPHSM culture medium. Extracellular (clear grey), intracellular (dark grey) activity (a), and …

Web1 Mar 2024 · The strains BJ3505 1 Suc2 and BJ3505 were transformed with. plasmids YEpFLAG-1, YEp MEL1, and YEp MEL1 His, by a. modiﬁcation of the lithium acetate method (Chen et al., 1992). Web10 Oct 2024 · S. cerevisiae strain BJ3505. Diﬀerent constructions were designed to obtain the degr ee of puriﬁcation necessary for enzymatic characterization and to improve the …

Webstrains DKY6281 and BJ3505. The strain DKY6281 contains normal vacuolar proteases but lacks the major vacuolar phos-phatase encoded by the PHO8 gene. The strain BJ3505 …

Webacidocalcisome phosphate homeostasis Introduction Phosphate is a limiting factor for the growth of living organisms. It is mainly taken up by the cells as P i and incorporated in biological molecules, including ATP, nucleic acids, and phospholipids. second industry definitionWebVacuoles were isolated from yeast strains BJ3505 and DKY6281 as described (30). Large-scale frozen vacuole preparations of BJ3505 vacuoles were used for studies of … punk winter outfits second infantry division memorialWebSeasonal and developmental variations in the transcriptional activities induced by Maclura pomifera and Morus rubra extracts in S . cere 6 isiae strain BJ3505. (A) Transcriptional activity... punk with a camera merchWeb10 Oct 2024 · The aim of this work has been to optimize the recombinant production and further characterization of α-galactosidase of Saccharomyces cerevisiae. Results: The … second infectionWebThe Saccharomyces Genome Database (SGD) provides comprehensive integrated biological information for the budding yeast Saccharomyces cerevisiae along with search and analysis tools to explore these data, enabling the discovery of functional relationships between sequence and gene products in fungi and higher organisms. Explore SGD. second infection worse than firstWeb1 Dec 2003 · Batch fermentations were carried out to examine and compare the patterns of cell growth and sugar metabolism for the two recombinant S. cerevisiae BJ3505 strains, δXR and δXR/STH. As shown in Fig. 1, the expression of the STH gene did not make significant changes in glucose utilization, cell growth, ethanol accumulation, and xylitol … second infantry division unit awards