Thp1 isre
WebThe stimulator of interferon genes (STING) is a critical protein in the activation of the immune system in response to DNA. It can participate the inflammatory response process by modulating the inflammation-preferred translation program through the STING-PKR-like endoplasmic reticulum kinase (PERK)-eIF2α pathway or by inducing the secretion of … Web开馆时间：周一至周日7:00-22:30 周五 7:00-12:00; 我的图书馆
Thp1 isre
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Webof five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter, which is unresponsive to activators of the NF-κB or AP-1 pathways. As a result, THP1-Lucia™ … WebTHP1-Blue® ISG cells were specifically designed for monitoring the interferon signaling pathway in a physiologically relevant cell line. THP1-Blue® were derived from the human …
WebInvivoGen offers several engineered THP-1 cell lines: THP1 NF-κB cells were specifically designed for monitoring the NF-κB signal transduction pathway in a physiologically … WebThe CellSensor™ SIE-bla THP-1 cell line contains a beta-lactamase reporter gene under control of the Sis Inducible Element (SIE), stably integrated into THP-1 cells.To construct …
WebThe CellSensor™ SIE-bla THP-1 cell line contains a beta-lactamase reporter gene under control of the Sis Inducible Element (SIE), stably integrated into THP-1 cells.To construct this cell line, the SIE-bla construct was transduced into THP-1 cells by lentivirus.Flow cytometry was used to isolate cells responsive to Interferon Gamma (IFNγ). This cell line … WebMay 13, 2024 · THP1 DUAL cells (1 × 10 5 cells per ... cleared of residual cells by centrifugation and analysed for NF-κB and ISRE reporter activity according to the …
WebISRE promoter. Luciferase Expression ATCC® Number TIB-202-ISRE-LUC2™ Organism Homo sapiens Tissue/Disease Source Acute Monocytic Leukemia Product Description …
WebProgrammed death-1 (PD-1), an immunoinhibitory receptor, is upregulated in T cells, B cells, NKT cells, and monocytes upon activation. More specifically, T-cell-associated PD-1 is … thunderbolt 3 specificationsWebSep 16, 2024 · THP1 cells were left uninfected or infected with HSV-1 for the indicated times before endogenous coimmunoprecipitation and immunoblot analysis with the indicated antibodies. ... Reporter assays indicated that mutation of Y167, Y240, or Y245 of MITA abolished its ability to activate ISRE (SI Appendix, Fig. S6B). Previously, ... thunderbolt 3 surface book 2WebAll the THP1 cells (parent, MAVS, and STING KO cells) were seeded each at cells per well in a 100 μL complete growth medium containing 100 nM PMA (phorbol 12-myristate 13-acetate) in 96-well plates. At 72-hour postdifferentiation, the derived macrophages were treated with 0.5–4 μ M cryptolepine in a 100 μ L PMA-free complete growth medium. thunderbolt 3 technical drawingsWebTHP-1 ISRE-Luc2 IFN Stimulation Concentration (ng/mL) Fold Change 1 10 100 1000 0 10 20 30 40 50 THP-1 ISRE-Luc2 IFN Stimulation Concentration (ng/mL) Fold Change 0.1 1 10 … thunderbolt 3 switchWebNF-kB-SEAP and IRF-Lucia luciferase Reporter Monocytes. THP1-Dual™ cells were derived from the human THP-1 monocyte cell line by stable integration of two inducible reporter … thunderbolt 3 to 10 gigabit ethernet adapterWebHTRF Quantification of IFN-b, IL1-b, and TNF-a in THP1 cells THP1 WT-cells were seeded at 1,000,000 cells per well in 275µL RPMI supplemented with 10% FCS. After incubation at 37°C, 5% CO2, the cells were stimulated or not with 30µL of the STING agonist 2’3’ cGAMP used at 400µM final. Stimulation was performed for 24 hours, then either ... thunderbolt 3 to 10gbeアダプターWebTo show that USP19 targets TBK1 to negatively regulate the antiviral response, we performed an IFNB1 and interferon-stimulated response element (ISRE) promoter reporter assay. Overexpressing USP19 inhibited TBK1 and the upstream regulators DDX58/RIG-I and MAVS overexpression induced IFNB1 and ISRE promoter activity ( Figure 5(A-F )). thunderbolt 3 thinkpad dock