WebApr 12, 2024 · Samples were trimmed as paired end reads using Trimmomatic and aligned to the human genome (hg38) using HISAT2. FeatureCounts measured differentially expressed genes (DEG) based on the annotated human genome. EdgeR was then used to compare AngII treated samples to untreated samples for both the male and female HUVEC. WebApr 12, 2024 · The Metagenome library was prepared and sequenced on Illumina Novoseq 6000 platform paired-end sequencing (2 × 150 bp) from CytoGene® Shotgun metagenome ... reads less than 50 bp in size, and adapter content were trimmed using Trimmomatic version 0.39 (Bolger et al. 2014) with the parameters of PE, -phred33, ILLUMNACLIP ...
Trimmomatic - BITS wiki - Universiteit Gent
WebAug 12, 2024 · Description. Trimmomatic performs a variety of useful trimming tasks for illumina paired-end and single ended data.The selection of trimming steps and their … WebA total of 397,856,687 paired end reads were obtained for the 24 LMD samples. After QC, an average of 31.1±6.5 million paired-end reads per sample were kept, and 94.42% were uniquely mapped across the swine reference genome. Out of those, 80.63% were mapped in genes. A total of 14,381 genes were expressed in the swine mediastinal lymph node. red beaded ornaments
Proceedings of the 12th World Congress on Genetics Applied to …
WebTrimmomatic uses these extra pieces of information to detect small pieces of contaminating adapters in paired end data. This approach is called palindrome mode (see … WebJul 8, 2024 · Trimmomatic paired-end mode question . 0. Entering edit mode. 2.6 years ago. slin023 • 0 Hi, I have trimmomatic 0.39-1 installed via bioconda and I have read the … Webarguments ( str) – Trimmomatic arguments (threads, phred, etc.) (default: ‘’) input_dir ( str) – Path to a directory containing input data. Required if r1_file for single-end data or r1_file and r2_file for paired-end data is/are not specified (default: None) output_dir ( str) – Path to a directory, where output files are saved. red beaded pillow